Sains Malaysiana 53(9)(2024): 2045-2055

http://doi.org/10.17576/jsm-2024-5309-03

Kesan Jangkitan Virus Sauropus Yellowing (SaYV) kepada Anatomi Daun Sauropus androgynus(L.) Merr.

(Effects of Sauropus Yellowing Virus (SaYV) Infection on Leaf Anatomy of Sauropus androgynus (L.) Merr.)

NORAINI TALIP^1,*, RAJ KUMAR¹, HAMIDUN BUNAWAN², MOHAMAD RUZI ABDUL RAHMAN³, NORDAHLIA ABDULLAH SIAM⁴ & SYAZWANI BASIR¹

¹Jabatan Sains Biologi dan Bioteknologi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

²Institut Biologi Sistem (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Taman Botani Bangi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

⁴Forest Research Institute of Malaysia (FRIM), 42200 Kepong, Selangor, Malaysia

Diserahkan: 24 April 2024/Diterima: 5 Julai 2024

Abstrak

Kajian anatomi perbandingan daun dilakukan ke atas daun Sauropus androygnus yang dijangkiti virus Sauropus yellowing (SaYV). Objektif kajian ialah untuk mengenal pasti ciri anatomi daun yang dijangkiti virus SaYV dan daun sihat (kawalan) serta menentukan ciri anatomi yang berubah dengan jangkitan virus SaYV. Kajian melibatkan keratan rentas lamina, petiol dan tepi daun menggunakan mikrotom gelongsor, teknik penjernihan untuk peruratan dan siatan epidermis untuk epidermis adaksial dan abaksial daun. Hasil kajian menunjukkan pengurangan peratusan pigmen klorofil pada lamina dan tepi daun, hiperplasia tisu vaskular pada tulang daun dan petiol, hiperplasia sel kolenkima dan pertambahan kepadatan hablur drus pada berkas vaskular lamina, tulang daun dan tisu mesofil. Terdapat kerosakan pada urat sekunder dan tertier daun akibat jangkitan virus. Hasil cerapan pada epidermis abaksial daun dijangkiti menunjukkan ketumpatan dan indeks stoma (SI) yang rendah berbanding daun sihat. Kajian ini membuktikan berlaku pengubahsuaian ciri anatomi S. androygnus akibat jangkitan virus SaYV sebagai respons terhadap jangkitan virus. Hasil kajian dapat memberi manfaat kepada pengusaha pertanian dan boleh menjadi rujukan dalam pengurusan penyakit pertanian terutama untuk kawalanpenyakit pada awal jangkitan virus.

Kata kunci: Bahan ergastik; cekur manis; mekanisme pertahanan tumbuhan; patogen tumbuhan; simptomatik

Abstract

A comparative leaf anatomy study was performed on Sauropus androygnus leaves infected with Sauropus yellowing virus (SaYV). The objective of the study was to identify the anatomical features of leaves infected with SaYV virus and healthy leaves (control), as well as to determine the anatomical features that change with SaYV virus infection. The study involved cross-sectioning of the lamina, petiole, and leaf margin using a sliding microtome, clearing techniques for leaf venation, and epidermal peels for the adaxial and abaxial epidermis. The results of the study showed a reduction in the percentage of chlorophyll pigment on the lamina and leaf margin, vascular tissue hyperplasia on the leaf midrib and petiole, collenchyma cell hyperplasia, and an increase in the density of druses on the vascular bundles of the lamina, leaf midrib, and mesophyll tissue. There is damage to secondary and tertiary leaf veins due to virus infection. Observation results on the abaxial epidermis of infected leaves show a low stomatal density and index (SI) compared to healthy leaves. This study proves that there is a modification of the anatomical features of S. androygnus due to SaYV virus infection in response to the virus infection. The results of the study can benefit agricultural operators and can be a reference in the management of agricultural diseases, especially for disease control at the beginning of virus infection.

Keywords: Cekur manis; ergastic substance; plant defense mechanism; plant pathogen; symptomatic

RUJUKAN

Agrawal, S.K., Karthikeyan, V., Parthiban, P. & Nandhini, R. 2014. Multivitamin plant: pharmacognostical standardization and phytochemical profile of its leaves. Journal of Pharmacy Research 8(7): 920-925.

Andarwulan, N., Batari, R., Sandrasari, D.A., Bolling, B. & Wijaya, H. 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chemistry 121(4): 1231-1235.

Andersen, E.J., Ali, S., Byamukama, E., Yen, Y. & Nepal, M.P. 2018. Disease resistance mechanisms in plants. Genes 9(7): 339.

Balachandran, S., Hurry, V., Kelley, S., Osmond, C., Robinson, S., Rohozinski, J., Seaton, G. & Sims, D. 1997. Concepts of plant biotic stress. Some insights into the stress physiology of virus‐infected plants, from the perspective of photosynthesis. Physiologia Plantarum 100(2): 203-213.

Bhaskar, A., Ramesh, K. & Rajeshwari, S. 2009. Wound healing profile of Sauropus androgynus in Wistar rats. Journal of Natural Remedies 9(2): 159-164.

Brisson, L.F., Tenhaken, R. & Lamb, C. 1994. Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. The Plant Cell 6(12): 1703-1712.

Bunawan, H., Bunawan, S.N., Baharum, S.N. & Noor, N.M. 2015. Sauropus androgynus (L.) Merr. induced bronchiolitis obliterans: From botanical studies to toxicology. Evidence-Based Complementary and Alternative Medicine 2015: 714158.

Casson, S.A. & Hetherington, A.M. 2010. Environmental regulation of stomal development. Current Opinion in Plant Biology 13(1): 90-95.

Cuming, A.C. & Stevenson, S.R. 2015. From pond slime to rain forest: The evolution of ABA signalling and the acquisition of dehydration tolerance. New Phytologist 206(1): 5-7.

Das, P.P., Lin, Q. & Wong, S.M. 2019. Comparative proteomics of Tobacco mosaic virus-infected Nicotiana tabacum plants identified major host proteins involved in photosystems and plant defence. Journal of Proteomics 194: 191-199.

El-Banna, O.H.M., Awad, M., Abbas, M., Waziri, H. & Darwish, H. 2014. Anatomical and ultrastructural changes in tomato and grapevine leaf tissues infected with Tomato ringspot virus. Egyptian Journal Virology 11: 102-111.

Fereres, A. & Raccah, B. 2015. Plant Virus Transmission by Insects. Chichester: John Wiley & Sons. hlm. 1-12.

Franceschi, V.R. & Horner, H.T. 1980. Calcium oxalate crystals in plants. The Botanical Review 46(4): 361-427.

Franceschi, V.R. & Nakata, P.A. 2005. Calcium oxalate in plants: Formation and function. Annual Review of Plant Biology 56: 41-71.

Funayama-Noguchi, S. 2001. Ecophysiology of virus-infected plants: A case study of Eupatorium makinoi infected by geminivirus. Plant Biology 3(03): 251-262.

Funayama-Noguchi, S. & Terashima, I. 2006. Effects of Eupatorium yellow vein virus infection on photosynthetic rate, chlorophyll content and chloroplast structure in leaves of Eupatorium makinoi during leaf development. Functional Plant Biology 33(2): 165-175.

Gilbertson, R.L. & Lucas, W.J. 1996. How do viruses traffic on the ‘vascular highway’? Trends in Plant Science 1(8): 250-251.

Ghosh, S., Kanakala, S., Lebedev, G., Kontsedalov, S., Silverman, D., Alon, T., Mor, N., Sela, N., Luria, N. & Dombrovsky, A. 2019. Transmission of a new polerovirus infecting pepper by the whitefly Bemisia tabaci. Journal of Virology 93(15): 488-419.

Glazebrook, J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Review of Phytopathology 43: 205-227.

Gouveia, B.C., Calil, I.P., Machado, J.P.B., Santos, A.A. & Fontes, E.P. 2017. Immune receptors and co-receptors in antiviral innate immunity in plants. Frontiers in Microbiology 7: 2139.

Halim, V., Vess, A., Scheel, D. & Rosahl, S. 2006. The role of salicylic acid and jasmonic acid in pathogen defence. Plant Biology 8(03): 307-313.

Jeandet, P. 2015. Phytoalexins: Current progress and future prospects. Molecules 20(2): 2770-2774.

Jyosthna, M., Reddy, N.E., Chalam, T. & Reddy, G. 2004. Morphological and biochemical characterization of Phaeoisariopsis personata resistant and susceptible cultivars of groundnut (Arachis hypogaea). Plant Pathology Bulletin 13(4): 243-250.

Kang, Z. & Buchenauer, H. 2003. Immunocytochemical localization of cell wall‐bound thionins and hydroxyproline‐rich glycoproteins in Fusarium culmorum‐infected wheat spikes. Journal of Phytopathology 151(3): 120-129.

Katayama, H., Fujibayashi, Y., Nagaoka, S. & Sugimura, Y. 2007. Cell wall sheath surrounding calcium oxalate crystals in mulberry idioblasts. Protoplasma 231(3): 245-248.

Knierim, D., Maiss, E., Menzel, W., Winter, S. & Kenyon, L. 2015. Characterization of the complete genome of a novel polerovirus infecting Sauropus androgynus in Thailand. Journal of Phytopathology 163(9): 695-702.

Knierim, D., Tsai, W., Maiss, E. & Kenyon, L. 2014. Molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. Archives of Virology 159(6): 1459-1465.

Lai, R.S., Chiang, A.A., Wu, M.T., Wang, J.S., Lai, N.S., Lu, J.Y., Ger, L.P. & Roggli, V. 1996. Outbreak of bronchiolitis obliterans associated with consumption of Sauropus androgynus in Taiwan. The Lancet 348(9020): 83-85.

Lake, J.A., Quick, W., Beerling, D.J. & Woodward, F.I. 2001. Signals from mature to new leaves. Nature 411: 154.

Leszczuk, A., Szczuka, E., Stanisławek, K., Mazurkiewcz, I. & Kasprzyk, A. 2014. Calcium oxalate crystals in the stem of Sida hermaphrodita (L.) Rusby (Malvaceae). Annales Universitatis Mariae Curie-Sklodowska Sectio C–Biologia 69(1). http://dx.doi.org/10.2478/umcsbio-2013-0003

Lin, L., Hsiao, Y. & Kuo, C.G. 2009. Discovering Indigenous Ttreasures: Promising Indigenous Vegetables from Around the World. Taiwan.: AVRDC- The World Vegetable Center Publication.

Liu, Q., Luo, L. & Zheng, L. 2018. Lignins: Biosynthesis and biological functions in plants. International Journal of Molecular Sciences 19(2): 335-351.

Mahajan, M. & Dhillon, M. 2003. Relation of leaf epidermal characteristics with susceptibility and resistance of potato (Solanum tuberosum) to late blight disease (Phytophthora infestans). Indian Journal of Agricultural Science 73(12): 656-660.

Mclachlan, D.H., Kopischke, M. & Robatzek, S. 2014. Gate control: Guard cell regulation by microbial stress. New Phytologist 203(4): 1049-1063.

Melotto, M., Underwood, W., Koczan, J., Nomura, K. & He, S.Y. 2006. Plant stoma function in innate immunity against bacterial invasion. Cell 126(5): 969-980.

Murray, R.R., Emblow, M.S., Hetherington, A.M. & Foster, G.D. 2016. Plant virus infections control stomal development. Scientific Reports 6: 34507.

Nakata, P.A. 2003. Advances in our understanding of calcium oxalate crystal formation and function in plants. Plant Science 164(6): 901-909.

Noraini, T., Nur Syahirah, M. & Mohamad Ruzi, A.R. 2022. Histologi dan Teknik Pengawetan Tumbuhan. Bangi: Universiti Kebangsaan Malaysia.

Noraini, T., Mohamad Ruzi, A.R. & Muhammad Amirul Aiman, A.J. 2019. Anatomi dan Mikroskopik Tumbuhan. Bangi: Universiti Kebangsaan Malaysia.

Padmavathi, P. & Rao, M.P. 1990. Nutritive value of Sauropus androgynus leaves. Plant Foods for Human Nutrition 40(2): 107-113.

Palanisamy, P., Michael, P.I. & Krishnaswamy, M. 2009. Physiological response of Yellow vein mosaic virus-infected bhendi [Abelmoschus esculentus] leaves. Physiological and Molecular Plant Pathology 74(2): 129-133.

Pattar, P.V. & Jayaraj, M. 2012. Pharmacognostic and phytochemical investigation of Sida cordifolia L. threatened medicinal herb. International Journal of Pharmacy and Pharmaceutical Sciences 4(1): 114-117.

Pillitteri, L.J. & Torii, K.U. 2012. Mechanisms of stomal development. Annual Review of Plant Biology 63: 591-614.

Radwan, D.E.M., Lu, G., Fayez, K.A. & Mahmoud, S.Y. 2008. Protective action of salicylic acid against bean yellow mosaic virus infection in Vicia faba leaves. Journal of Plant Physiology 165(8): 845-857.

Reusche, M., Truskina, J., Thole, K., Nagel, L., Rindfleisch, S., Braus-Stromeyer, S.A., Braus, G.H., Teichmann, T. & Lipka, V. 2014. Infections with the vascular pathogens Verticillium longisporum and Verticillium dahliae induce distinct disease symptoms and differentially affect drought stress tolerance of Arabidopsis thaliana. Environmental and Experimental Botany 108: 23-37.

Rodrigues, L.M.R., Queiroz-Voltan, R.B. & Guerreiro, O. 2015. Anatomical changes on coffee leaves infected by Pseudomonas syringae pv. garcae. Summa Phytopathologica 41: 256-261.

Sai, K. & Srividya, N. 2002. Blood glucose lowering effect of the leaves of Tinospora cordifolia and Sauropus androgynus in diabetic subjects. Journal of Natural Remedies 2(1): 28-32.

Selvi, V.S. & Bhaskar, A. 2012. Characterization of anti-inflammatory activities and antinociceptive effects of papaverine from Sauropus androgynus (L.) Merr. Global. Journal of Pharmacology 6(3): 86-192.

Shailasree, S., Kini, K.R., Deepak, S., Kumudini, B. & Shetty, H.S. 2004. Accumulation of hydroxyproline-rich glycoproteins in pearl millet seedlings in response to Sclerospora graminicola infection. Plant Science 167(6): 1227-1234.

Simon, N.M., Sugisaka, J., Honjo, M.N., Tunstad, S.A., Tunna, G., Kudoh, H. & Dodd, A.N. 2020. Altered stomal patterning accompanies a trichome dimorphism in a natural population of Arabidopsis. Plant Direct 4(9): e00262.

Soka, S., Alam, H., Boenjamin, N., Agustina, T.W. & Suhartono, M.T. 2010. Effect of Sauropus androgynus leaf extracts on the expression of prolactin and oxytocin genes in lactating balb/c mice. Lifestyle Genomics 3(1): 31-36.

Stenglein, S.A., Arambarri, A.M., Sevillano, M.D.C.M. & Balatti, P.A. 2005. Leaf epidermal characters related with plant's passive resistance to pathogens vary among accessions of wild beans Phaseolus vulgaris var. aborigineus (Leguminosae–Phaseoleae). Flora-Morphology, Distribution, Functional Ecology of Plants 200(3): 285-295.

Vorwerk, S., Somerville, S. & Somerville, C. 2004. The role of plant cell wall polysaccharide composition in disease resistance. Trends in Plant Science 9(4): 203-209.

Waheeda, A.W., Noraini, T., Basir, S., Muhamad Afiq, A., Mohd Faiz, M.S. & Bunawan, H. 2022. Disease development and discovery of anatomically resistant features towards bacterial leaf streak in rice. Agriculture 12(5): 629. https://www.mdpi.com/2077-0472/12/5/629

Walker, P.J., Siddell, S.G., Lefkowitz, E.J., Mushegian, A.R., Adriaenssens, E.M., Alfenas-Zerbini, P., Davison, A.J., Dempsey, D.M., Dutilh, B.E., García, M.L., Harrach, B., Harrison, R.L., Hendrickson, R.C., Junglen, S., Knowles, N.J., Krupovic, M., Kuhn, J.H., Lambert, A.J., Łobocka, M., Nibert, M.L., Oksanen, H.M., Orton, R.J., Robertson, D.L., Rubino, L., Sabanadzovic, S., Simmonds P, Smith, D.B., Suzuki, N., Van Dooerslaer, K., Vandamme, A.M., Varsani, A. & Zerbini, F.M. 2021. Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature Ratified by the International Committee on Taxonomy of Viruses (2021). Archives of Virology166(9): 2633-2648.

White, P.J. 2012. Long-distance transport in the xylem and phloem. In Marschner’s Mineral Nutrition of Higher Plants, (Edisi-3), disunting oleh Marschner, P. Amsterdam: Academic Press. hlm. 49-70.

Yusop, M.S.M., Lanisa, N., Talip, N., Mat Saad, M.F., Abas, A., Sarian, M.N. & Bunawan, H. 2023. The complete genome sequence, molecular detection, and anatomical analysis of Hibiscus chlorotic ringspot virus infecting Hibiscus rosa-sinensis in Peninsular Malaysia. Horticulturae 9(5): 569.

Zhang, B.D., Cheng, J.X., Zhang, C.F., Bai, Y.D., Liu, W.Y., Li, W., Koike, K., Akihisa, T., Feng, F. & Zhang, J. 2020. Sauropus androgynus L. Merr. a phytochemical, pharmacological and toxicological review. Journal of Ethnopharmacology 257: 112778.

*Pengarang untuk surat-menyurat; email: ntalip@ukm.edu.my